Tristetraprolin down-regulates IL-17 through mRNA destabilization

FEBS Lett. 2012 Jan 2;586(1):41-6. doi: 10.1016/j.febslet.2011.11.021. Epub 2011 Nov 28.

Abstract

An excess of interleukin 17 (IL-17) may contribute to chronic inflammatory disorders, but mechanisms that regulate IL-17 in immune cells are unclear. Here we report that tristetraprolin (TTP) inhibits IL-17 production in human T cell lines. Overexpression of TTP decreased the expression of IL-17. Conversely, TTP inhibition by siRNA increased IL-17 production. IL-17 mRNA contains eight AREs within its 3'UTR. TTP bound directly to the IL-17 mRNA 3'UTR at a location between the fourth and seventh AREs and enhanced decay of IL-17 transcripts. These results suggest that TTP could control IL-17-mediated inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Base Sequence
  • Binding Sites
  • Cell Line
  • Down-Regulation
  • Gene Expression Regulation
  • Humans
  • Interleukin-17 / genetics*
  • Interleukin-17 / metabolism
  • Molecular Sequence Data
  • RNA Stability*
  • RNA, Small Interfering
  • T-Lymphocytes / metabolism
  • Tristetraprolin / genetics
  • Tristetraprolin / metabolism*

Substances

  • 3' Untranslated Regions
  • Interleukin-17
  • RNA, Small Interfering
  • Tristetraprolin
  • ZFP36 protein, human