Synthesis of a dual-labeled probe of dimethyl lithospermate B with photochemical and fluorescent properties

Molecules. 2011 Nov 28;16(12):9886-99. doi: 10.3390/molecules16129886.

Abstract

Dimethyl lithosermate B (DLB) is a highly potent natural antioxidant and antidiabetic polyphenol with unknown mode of action. To determine its cellular targets, a photochemical and fluorescent dimethyl lithopermate B probe was designed and efficiently synthesized. The dual-labeled chemical probe for biological application was evaluated by UV and fluorescence to determine its electrochemical absorption and emission properties. This probe could be valuable for investigating ligand-protein interactions and subcellular localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drugs, Chinese Herbal / chemical synthesis*
  • Drugs, Chinese Herbal / chemistry
  • Molecular Probes / chemical synthesis*
  • Photoaffinity Labels / metabolism*
  • Photochemical Processes*
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Ultraviolet Rays

Substances

  • Drugs, Chinese Herbal
  • Molecular Probes
  • Photoaffinity Labels
  • dimethyl lithospermate B