Abstract
The gammaretroviruses xenotropic murine leukemia virus (MLV)-related virus (XMRV) and MLV have been reported to be more prevalent in plasma and peripheral blood mononuclear cells of chronic fatigue syndrome (CFS) patients than in healthy controls. Here, we report the complex analysis of whole blood and plasma samples from 58 CFS patients and 57 controls from Canada for the presence of XMRV/MLV nucleic acids, infectious virus, and XMRV/MLV-specific antibodies. Multiple techniques were employed, including nested and qRT-PCR, cell culture, and immunoblotting. We found no evidence of XMRV or MLV in humans and conclude that CFS is not associated with these gammaretroviruses.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Anti-Idiotypic / blood*
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Antibodies, Viral / blood*
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Blotting, Western
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Canada
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Case-Control Studies
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DNA, Viral / genetics*
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Fatigue Syndrome, Chronic / blood
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Fatigue Syndrome, Chronic / immunology*
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Fatigue Syndrome, Chronic / virology*
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Female
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Humans
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Leukemia Virus, Murine / genetics
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Leukemia Virus, Murine / isolation & purification
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Male
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Middle Aged
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Retroviridae Infections / diagnosis
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Retroviridae Infections / virology*
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Virus Infections / diagnosis
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Tumor Virus Infections / virology*
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Xenotropic murine leukemia virus-related virus / genetics
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Xenotropic murine leukemia virus-related virus / isolation & purification
Substances
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Antibodies, Anti-Idiotypic
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Antibodies, Viral
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DNA, Viral
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RNA, Messenger