Protective effect of ginseng sapogenins against 2,2'-azobis (1-aminopropane) dihydrochloride (AAPH)-induced LLC-PK₁ cell damage

Bioorg Med Chem Lett. 2012 Jan 1;22(1):634-7. doi: 10.1016/j.bmcl.2011.10.063. Epub 2011 Oct 24.

Abstract

The effect of ginseng sapogenins, aglycone parts of ginsenosides, against oxidative damage by radical generator, 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), in renal epithelial LLC-PK(1) cells was investigated to identify the structural characteristics of sapogenins to have renoprotective effects. Of the tested sapogenins, Δ(20(21))-protopanaxatriol showed the strongest protective effect against the AAPH-induced LLC-PK(1) cell damage. Based on the structure and stronger activity of Δ(20(21))-protopanaxatriol than the other sapogenins, the hydroxyl group in C-6 and double bond in C-20(21) position were important for renoprotective effect of sapogenin against oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidines / adverse effects*
  • Animals
  • Cell Line
  • Chemistry, Pharmaceutical / methods
  • Chromatography / methods
  • Chromatography, High Pressure Liquid / methods
  • Drug Design
  • Epithelial Cells / metabolism
  • Kidney / metabolism
  • LLC-PK1 Cells
  • Magnetic Resonance Spectroscopy / methods
  • Models, Biological
  • Oxidative Stress
  • Panax / metabolism*
  • Sapogenins / chemistry
  • Sapogenins / metabolism*
  • Swine
  • Time Factors

Substances

  • Amidines
  • Sapogenins
  • 2,2'-azobis(2-amidinopropane)