Virulence factors and TEM-type β-lactamases produced by two isolates of an epidemic Klebsiella pneumoniae strain

Antimicrob Agents Chemother. 2012 Feb;56(2):1101-4. doi: 10.1128/AAC.05079-11. Epub 2011 Nov 21.

Abstract

Two Klebsiella pneumoniae isolates of the same strain, identified in Poland, produced either TEM-47 or TEM-68, which differed by the Arg275Leu substitution. They harbored a few virulence factors, including an iron-chelating factor and capsule overproduction, suggesting that these factors were sufficient to enhance their nosocomial potency. TEM-68 and TEM-47 had similar enzymatic activities, but TEM-68 was less susceptible to inhibitors than TEM-47. These results confirm the role of the Arg275Leu substitution in the evolution of TEM enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Cross Infection / epidemiology
  • Cross Infection / microbiology
  • Cross Infection / transmission*
  • Epidemics*
  • Humans
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology
  • Klebsiella Infections / transmission
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / pathogenicity*
  • Microbial Sensitivity Tests
  • Mutation
  • Poland / epidemiology
  • Virulence
  • Virulence Factors / biosynthesis*
  • Virulence Factors / genetics
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / genetics

Substances

  • Virulence Factors
  • beta-lactamase TEM-47
  • beta-lactamase TEM-68
  • beta-Lactamases