Phosphopeptide-dependent labeling of 14-3-3 ζ proteins by fusicoccin-based fluorescent probes

Michiko Takahashi; Akie Kawamura; Nobuo Kato; Tsuyoshi Nishi; Itaru Hamachi; Junko Ohkanda

doi:10.1002/anie.201106995

Phosphopeptide-dependent labeling of 14-3-3 ζ proteins by fusicoccin-based fluorescent probes

Angew Chem Int Ed Engl. 2012 Jan 9;51(2):509-12. doi: 10.1002/anie.201106995. Epub 2011 Nov 21.

Authors

Michiko Takahashi¹, Akie Kawamura, Nobuo Kato, Tsuyoshi Nishi, Itaru Hamachi, Junko Ohkanda

Affiliation

¹ The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.

PMID: 22105970
DOI: 10.1002/anie.201106995

Abstract

Fluorescent combination: Cell-penetrating probes derived from the diterpene fusicoccin can form ternary complexes with 14-3-3 proteins and phosphopeptide ligands, whereupon the probes site-specifically attach a fluorescent tag onto the surface of the 14-3-3 proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

14-3-3 Proteins / analysis*
14-3-3 Proteins / chemistry
Cell Line
Fluorescent Dyes / chemistry*
Glycosides / chemistry*
Humans
Models, Molecular
Phosphopeptides / chemistry*
Staining and Labeling

Substances

14-3-3 Proteins
Fluorescent Dyes
Glycosides
Phosphopeptides
fusicoccin