An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy

Biophys J. 2011 Nov 16;101(10):L50-2. doi: 10.1016/j.bpj.2011.10.022. Epub 2011 Nov 15.

Abstract

A novel, to our knowledge, in situ photoirradiation system for solid-state NMR measurements is improved and demonstrated to successfully identify the M-photointermediate of pharaonis phoborhodopsin (ppR or sensory rhodopsin II), that of the complex with transducer (ppR/pHtrII), and T204A mutant embedded in a model membrane. The (13)C NMR signals from [20-(13)C]retinal-ppR and ppR/pHtrII revealed that multiple M-intermediates with 13-cis, 15-anti retinal configuration coexisted under the continuously photoirradiated condition. NMR signals observed from the photoactivated retinal provide insights into the process of photocycle in the ppR/pHtrII complex.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Halorhodopsins / metabolism*
  • Halorhodopsins / radiation effects*
  • Light*
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Mutant Proteins / metabolism
  • Mutant Proteins / radiation effects
  • Sensory Rhodopsins / metabolism*
  • Sensory Rhodopsins / radiation effects*

Substances

  • Halorhodopsins
  • Mutant Proteins
  • Sensory Rhodopsins
  • sensory rhodopsin II protein, archaeal