Abstract
Cisplatin (DDP) is widely used in lung cancer chemotherapy. However, cisplatin resistance represents a major obstacle in effective clinical treatment. This study aims to investigate whether Newcastle disease virus (NDV) exhibits an oncolytic effect on cisplatin-resistant A549 lung cancer cells. We found that NDV induced A549/DDP cell apoptosis via the caspase pathway, particularly involving caspase-9, while the mitogen-activated protein kinase (MAPK) and Akt pathways also contributed to apoptotic induction. Furthermore, NDV displayed oncolytic effects in a mouse A549/DDP lung cancer model. Collectively, our data indicate that NDV could overcome the cisplatin resistance in lung cancer cells in vitro and in vivo.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenocarcinoma / metabolism
-
Adenocarcinoma / pathology
-
Adenocarcinoma / therapy*
-
Adenocarcinoma / virology
-
Adenocarcinoma of Lung
-
Animals
-
Antineoplastic Agents / pharmacology*
-
Apoptosis*
-
Caspase 9 / metabolism
-
Cell Line, Tumor
-
Cisplatin / pharmacology*
-
Drug Resistance, Neoplasm*
-
Female
-
Humans
-
Lung Neoplasms / metabolism
-
Lung Neoplasms / pathology
-
Lung Neoplasms / therapy*
-
Lung Neoplasms / virology
-
Mice
-
Mice, Inbred BALB C
-
Mitogen-Activated Protein Kinases / metabolism
-
Newcastle disease virus / growth & development
-
Newcastle disease virus / pathogenicity*
-
Oncolytic Virotherapy*
-
Oncolytic Viruses / growth & development
-
Oncolytic Viruses / pathogenicity*
-
Proto-Oncogene Proteins c-akt / metabolism
-
Signal Transduction
-
Time Factors
-
Virus Replication
-
Xenograft Model Antitumor Assays
Substances
-
Antineoplastic Agents
-
Proto-Oncogene Proteins c-akt
-
Mitogen-Activated Protein Kinases
-
CASP9 protein, human
-
Caspase 9
-
Cisplatin