Novel multi-component enzyme machinery in lactic acid bacteria catalyzing C=C double bond migration useful for conjugated fatty acid synthesis

Biochem Biophys Res Commun. 2011 Dec 9;416(1-2):188-93. doi: 10.1016/j.bbrc.2011.11.022. Epub 2011 Nov 10.

Abstract

Linoleic acid isomerase was identified as a multi-component enzyme system that consists of three enzymes that exist in both the membrane and soluble fractions of Lactobacillus plantarum. One enzyme (CLA-HY) is present in the membrane fraction, while two enzymes (CLA-DH and CLA-DC) exist in the soluble fraction. Three Escherichia coli transformants expressing CLA-HY, CLA-DH, and CLA-DC were constructed. Conjugated linoleic acid (CLA) and 10-hydroxy-12-octadecenoic acid were generated from linoleic acid only when all these three E. coli transformants were used as catalysts simultaneously. CLA-HY catalyzed the hydration reaction, a part of linoleic acid isomerization, to produce 10-hydroxy-12-octadecenoic acid. This multi-component enzyme system required oxidoreduction cofactors such as NADH and FAD. This is the first report to reveal enzymes genes and the elaborate machinery that synthesizes CLA, especially an important isomer of cis-9, trans-11-CLA, in lactic acid bacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification
  • Catalysis
  • Cloning, Molecular
  • Enzymes / chemistry*
  • Enzymes / genetics
  • Enzymes / isolation & purification
  • Lactobacillus plantarum / enzymology*
  • Lactobacillus plantarum / genetics
  • Linoleic Acids, Conjugated / biosynthesis*

Substances

  • Bacterial Proteins
  • Enzymes
  • Linoleic Acids, Conjugated
  • cis-9, trans-11-conjugated linoleic acid