Accuracy of GFR predictive equations in renal transplantation: validation of a new turbidimetric cystatin C assay on Architect c8000

A S Bargnoux; A C Servel; L Piéroni; A M Dupuy; S Badiou; V Garrigue; G Mourad; J P Cristol; SFBC Biology of renal function and renal failure working group

doi:10.1016/j.clinbiochem.2011.10.018

Accuracy of GFR predictive equations in renal transplantation: validation of a new turbidimetric cystatin C assay on Architect c8000

Clin Biochem. 2012 Jan;45(1-2):151-3. doi: 10.1016/j.clinbiochem.2011.10.018. Epub 2011 Nov 6.

Authors

A S Bargnoux¹, A C Servel, L Piéroni, A M Dupuy, S Badiou, V Garrigue, G Mourad, J P Cristol; SFBC Biology of renal function and renal failure working group

Affiliation

¹ Department of Biochemistry, University of Montpellier 1, Montpellier, France.

PMID: 22079396
DOI: 10.1016/j.clinbiochem.2011.10.018

Abstract

Objectives: To evaluate the Sentinel-PETIA cystatin C on Architect c8000 analyzer.

Design and methods: We assessed analytical performances and clinical relevance by comparison with a reference isotopic method in kidney transplant recipients.

Results: This assay exhibited reliable precision and was close to the non standardized Siemens-PENIA method. All tested equations allowed reliable assessment of GFR.

Conclusions: Cystatin C improved GFR determination at the critical level of 60 mL/min/1.73 m². New formulas might be necessary after IFCC standardization.

MeSH terms

Adult
Aged
Calibration
Clinical Laboratory Techniques
Creatinine / blood
Cystatin C / blood*
Female
Glomerular Filtration Rate*
Humans
Kidney Failure, Chronic / therapy
Kidney Function Tests
Kidney Transplantation / methods*
Male
Middle Aged
Nephelometry and Turbidimetry / methods*
Particle Size
Predictive Value of Tests
Reference Standards
Reproducibility of Results

Substances

Cystatin C
Creatinine