Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy

Xianli Liu; Jinqiu Jing; Shengfang Li; Guodong Zhang; Tao Zou; Xianyou Xia; Weidong Huang

doi:10.1016/j.chemosphere.2011.10.019

Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy

Chemosphere. 2012 Jan;86(2):198-201. doi: 10.1016/j.chemosphere.2011.10.019. Epub 2011 Nov 8.

Authors

Xianli Liu¹, Jinqiu Jing, Shengfang Li, Guodong Zhang, Tao Zou, Xianyou Xia, Weidong Huang

Affiliation

¹ Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Huangshi Institute of Technology, Huangshi 435003, PR China.

PMID: 22071375
DOI: 10.1016/j.chemosphere.2011.10.019

Abstract

A synchronous fluorescence spectroscopy (SFS) method was developed for determination of pyrene in the gills of exposed fish. The wavelength differences (Δλ) of 50 nm was maintained between excitation and emission wavelengths and it was found to be suitable for the effective determination of pyrene in fish gills; the peak were observed at λ(ex) 334.5 nm. Linear relationships between SFS intensity and the concentration of pyrene in n-hexane solution were established. It was demonstrated that the SFS method was effective, simple, and less expensive, providing an attractive alternative for the rapid analysis of pyrene in fish gills.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Environmental Exposure
Environmental Monitoring / methods*
Fishes
Gills / chemistry*
Hexanes / chemistry
Pyrenes / analysis*
Spectrometry, Fluorescence*

Substances

Hexanes
Pyrenes
n-hexane
pyrene