Abstract
In this study, intestinal epithelial cells (IEC)-6 were cultured in different concentration extracts of Mg-6Zn alloys for different time periods. We studied the indirect effects of Mg-6Zn alloys on cell cycle of IEC-6 cells. The cell cycle of IEC-6 cells was measured using flow cytometry. And, the cell cycle of IEC-6 cells was evaluated by investigating the expression of cyclin D1, CDK4, and P21 using real-time polymerase chain reaction (PCR) and Western blotting tests. It was found that the IEC-6 cells displayed better cell functions in 20% extract of the Mg-6Zn alloy extracts, compared to the 100% or 60% extract. The in vitro results indicated that the conspicuous alkaline environment that is a result of rapid corrosion of Mg-6Zn alloys is disadvantageous to cell cycle of IEC-6 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Absorbable Implants / adverse effects*
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Alloys / toxicity
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Animals
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Cell Cycle / drug effects*
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Cell Cycle / genetics
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Cell Cycle / physiology
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Cell Line
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Corrosion
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Cyclin D1 / genetics
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Cyclin D1 / metabolism
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Cyclin-Dependent Kinase 4 / genetics
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Cyclin-Dependent Kinase 4 / metabolism
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Cyclin-Dependent Kinase Inhibitor p21 / genetics
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Cyclin-Dependent Kinase Inhibitor p21 / metabolism
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Gene Expression / drug effects
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Intestinal Mucosa / cytology
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Intestinal Mucosa / drug effects*
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Intestinal Mucosa / metabolism
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Magnesium / toxicity*
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Materials Testing
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
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Zinc / toxicity*
Substances
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Alloys
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Ccnd1 protein, rat
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Cdkn1a protein, rat
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Cyclin-Dependent Kinase Inhibitor p21
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Mg-6Zn alloy
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RNA, Messenger
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Cyclin D1
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Cdk4 protein, rat
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Cyclin-Dependent Kinase 4
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Magnesium
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Zinc