Objective: On basis of preliminary studies, to prepare the effective components group of Xiaoxuming decoction for anti-ischemic by combining chemical analysis with pharmacological activity screening.
Method: Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay; TBA method was used to determine mitochondrial lipid peroxidation.
Result: Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest.
Conclusion: 40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.