As a famous traditional Chinese medicine, ginseng's metabolism has been a hot spot in recent years. In this review, relevant literatures on the research progress of the metabolism of ginsenosides in vitro have been introduced. This review focuses on artifical juice metabolism, intestinal bacteria metabolism, microbial and enzymatic biotransformation of ginsenosides. Main metabolic pathways of ginsenosides in vitro are also analyzed, which will provide a reference for the systematic study on metabolism of ginsenosides.