A novel dendrimeric peptide induces high level neutralizing antibodies against classical swine fever virus in rabbits

Vet Microbiol. 2012 Apr 23;156(1-2):200-4. doi: 10.1016/j.vetmic.2011.10.012. Epub 2011 Oct 15.

Abstract

The amino acid sequence (TAVSPTTLR, 829-837aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B(4)) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B(4) elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B(4) may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing
  • Antibodies, Viral / immunology
  • Classical Swine Fever / immunology
  • Classical Swine Fever / prevention & control*
  • Classical Swine Fever Virus / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes, B-Lymphocyte / chemistry
  • Epitopes, B-Lymphocyte / immunology
  • Neutralization Tests
  • Rabbits
  • Swine
  • Vaccines, Subunit / chemistry
  • Vaccines, Subunit / immunology
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / chemistry
  • Viral Vaccines / immunology*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Epitopes, B-Lymphocyte
  • Vaccines, Subunit
  • Viral Envelope Proteins
  • Viral Vaccines