Abstract
Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC-SIGN/CD209 and HLA-DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti-inflammatory cytokine IL-10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage-fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages.
© 2011 John Wiley & Sons A/S.
Publication types
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Letter
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / metabolism
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Antigens, Differentiation, Myelomonocytic / metabolism
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Cell Adhesion Molecules / metabolism
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Cell Culture Techniques / methods*
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Cell Membrane / metabolism
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Cytoplasmic Vesicles / metabolism
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Dermis / cytology*
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Fibroblasts / cytology
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HLA-DR Antigens / metabolism
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Humans
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Interleukin-10 / metabolism
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Lectins, C-Type / metabolism
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Lipopolysaccharides / pharmacology
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Macrophages / cytology*
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Macrophages / drug effects
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Macrophages / metabolism
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Microspheres
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Monocytes / metabolism
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Phagocytosis / physiology
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Receptors, Cell Surface / metabolism
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Skin / cytology*
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Tissue Scaffolds
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Antigens, CD
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Antigens, Differentiation, Myelomonocytic
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CD163 antigen
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Cell Adhesion Molecules
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DC-specific ICAM-3 grabbing nonintegrin
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HLA-DR Antigens
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IL10 protein, human
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Lectins, C-Type
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Lipopolysaccharides
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Receptors, Cell Surface
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Tumor Necrosis Factor-alpha
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Interleukin-10