Quantification of hydroxyprolyl-glycine (Hyp-Gly) in human blood after ingestion of collagen hydrolysate

Fumihito Sugihara; Naoki Inoue; Masanori Kuwamori; Makoto Taniguchi

doi:10.1016/j.jbiosc.2011.09.024

Quantification of hydroxyprolyl-glycine (Hyp-Gly) in human blood after ingestion of collagen hydrolysate

J Biosci Bioeng. 2012 Feb;113(2):202-3. doi: 10.1016/j.jbiosc.2011.09.024. Epub 2011 Oct 29.

Authors

Fumihito Sugihara¹, Naoki Inoue, Masanori Kuwamori, Makoto Taniguchi

Affiliation

¹ Nitta Gelatin Inc., Peptide Division, 2-22 Futamata, Yao, Osaka 581-0024, Japan. fu-sugihara@nitta-gelatin.co.jp

PMID: 22040651
DOI: 10.1016/j.jbiosc.2011.09.024

Abstract

Plasma levels of prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly) in healthy volunteers (n=5) after ingestion of collagen hydrolysate were estimated by liquid chromatography-tandem mass spectrometry. The ratio of Hyp-Gly to Pro-Hyp was distributed in the range of 0.063-0.221. This is a first report for quantification of food-derived Hyp-Gly in human plasma.

MeSH terms

Adult
Collagen / administration & dosage*
Collagen / metabolism
Dipeptides / blood*
Eating
Humans
Hydrolysis
Male
Tandem Mass Spectrometry

Substances

Dipeptides
hydroxyprolyl-glycine
prolyl-4-hydroxyproline
Collagen