Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification

Virol J. 2011 Oct 31:8:489. doi: 10.1186/1743-422X-8-489.

Abstract

A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease / virology*
  • Foot-and-Mouth Disease Virus / classification*
  • Foot-and-Mouth Disease Virus / isolation & purification*
  • Molecular Diagnostic Techniques / methods*
  • Molecular Typing / methods*
  • Nucleic Acid Amplification Techniques / methods*
  • RNA, Viral / genetics
  • Reverse Transcription
  • Sensitivity and Specificity
  • Temperature
  • Time Factors

Substances

  • RNA, Viral