Two-dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines

Xi Chen; Margaret H MacDonald; Farooq Khan; Wesley M Garrett; Benjamin F Matthews; Savithiry S Natarajan

doi:10.1002/pmic.201100220

Two-dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines

Proteomics. 2011 Dec;11(24):4742-6. doi: 10.1002/pmic.201100220. Epub 2011 Nov 23.

Authors

Xi Chen¹, Margaret H MacDonald, Farooq Khan, Wesley M Garrett, Benjamin F Matthews, Savithiry S Natarajan

Affiliation

¹ USDA-ARS, Soybean Genomics and Improvement Laboratory, PSI, Beltsville, MD 20705, USA.

PMID: 22038885
DOI: 10.1002/pmic.201100220

Abstract

2-DE reference maps of Heterodera glycines were constructed. After in-gel digestion with trypsin, 803 spots representing 426 proteins were subsequently identified by LC-MS/MS. Proteins with annotated function were further categorized by Gene Ontology. The results showed that proteins involved in metabolic, developmental and biological regulation processes were the most abundant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Proteome / analysis
Proteomics / methods
Protozoan Proteins / analysis*
Tylenchoidea / metabolism*

Substances

Proteome
Protozoan Proteins