Purification of a new fungal mannose-specific lectin from Penicillium chrysogenum and its aphicidal properties

Fungal Biol. 2011 Nov;115(11):1093-9. doi: 10.1016/j.funbio.2011.06.010. Epub 2011 Jul 6.

Abstract

Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on SDS-PAGE gel. Purified PeCL fraction was delivered via artificial diet to Myzus persicae aphid and was demonstrated to be aphicidal at 0.1 % with higher toxic efficiency than a known mannose-binding lectin Concanavalin A (ConA). A fast and efficient way to purify PeCL and a potential use in pest control is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids / drug effects*
  • Fungal Proteins / chemistry
  • Fungal Proteins / isolation & purification*
  • Fungal Proteins / metabolism
  • Fungal Proteins / toxicity
  • Hemagglutination Tests
  • Insecticides / chemistry
  • Insecticides / isolation & purification*
  • Insecticides / metabolism
  • Insecticides / toxicity
  • Mannose-Binding Lectins / chemistry
  • Mannose-Binding Lectins / isolation & purification*
  • Mannose-Binding Lectins / metabolism
  • Mannose-Binding Lectins / toxicity
  • Penicillium chrysogenum / chemistry*
  • Penicillium chrysogenum / metabolism
  • Rabbits

Substances

  • Fungal Proteins
  • Insecticides
  • Mannose-Binding Lectins