Abstract
The in vivo analysis of the roles of splicing factors in regulating alternative splicing in animals remains a challenge. Using a microarray-based screen, we identified a Caenorhabditis elegans gene, tos-1, that exhibited three of the four major types of alternative splicing: intron retention, exon skipping, and, in the presence of U2AF large subunit mutations, the use of alternative 3' splice sites. Mutations in the splicing factors U2AF large subunit and SF1/BBP altered the splicing of tos-1. 3' splice sites of the retained intron or before the skipped exon regulate the splicing pattern of tos-1. Our study provides in vivo evidence that intron retention and exon skipping can be regulated largely by the identities of 3' splice sites.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing / genetics*
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Animals
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Base Sequence
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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DNA-Binding Proteins / metabolism*
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Exons*
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Gene Expression Regulation
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Gene Order
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Introns*
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Mutation / genetics
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RNA Splice Sites / genetics
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RNA Splicing Factors
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Ribonucleoproteins / genetics
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Ribonucleoproteins / metabolism*
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Transcription Factors / metabolism*
Substances
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Caenorhabditis elegans Proteins
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DNA-Binding Proteins
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RNA Splice Sites
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RNA Splicing Factors
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Ribonucleoproteins
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Transcription Factors
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UAF-1 protein, C elegans
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sfa-1 protein, C elegans