Direct, simultaneous measurement of liposome-encapsulated and released drugs in plasma by on-line SPE-SPE-HPLC

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3620-5. doi: 10.1016/j.jchromb.2011.10.004. Epub 2011 Oct 6.

Abstract

A method for the simultaneous measurement of liposome-encapsulated and released drugs in mouse plasma by on-line solid phase extraction (SPE)-SPE-HPLC with direct plasma injection was developed using a doxorubicin (DXR)-containing liposome formulation as the model drug. During SPE, the released DXR was extracted on the 1st restricted-access media (RAM) SPE column, whereas the liposomes were eluted. The eluted liposomes were collapsed on-line, and the released DXR was delivered to the 2nd RAM SPE column for extraction. The retained DXR on the SPE columns was analyzed via HPLC-fluorescent detector by switching the valves. The method was validated and applied to the pharmacokinetic study of DXR in mice after intravenous injection of DXR-containing liposomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates
  • Animals
  • Chromatography, High Pressure Liquid / methods*
  • Doxorubicin / administration & dosage
  • Doxorubicin / blood*
  • Doxorubicin / pharmacokinetics
  • Drug Stability
  • Equipment Design
  • Hydrogen-Ion Concentration
  • Liposomes / blood*
  • Liposomes / pharmacokinetics
  • Male
  • Methanol
  • Mice
  • Mice, Inbred BALB C
  • Reproducibility of Results
  • Solid Phase Extraction / methods*

Substances

  • Acetates
  • Liposomes
  • Doxorubicin
  • ammonium acetate
  • Methanol