A cell population structuring model to estimate recombinant strain growth in a closed system for subsequent search of the mode to increase protein accumulation during protealysin producer cultivation

Biofabrication. 2011 Dec;3(4):045006. doi: 10.1088/1758-5082/3/4/045006. Epub 2011 Oct 25.

Abstract

In this paper we have proposed a new structured population growth model, further developing a model previously proposed by the authors. Based on this model, optimal growth characteristics of the recombinant strain Escherichia coli BL-21 (DE3) [pProPlnHis(6)] were determined, which allowed us to increase the output of metalloproteinase by 300%. We have experimentally demonstrated the applicability of the new model to cell cultures with implanted plasmids and the potential practical use for an output increase of a wide variety of biosynthesis processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques / methods*
  • DNA, Recombinant / genetics
  • Escherichia coli / cytology*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / growth & development*
  • Escherichia coli Proteins / metabolism*
  • Genetic Engineering*
  • Human Umbilical Vein Endothelial Cells / cytology
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • Metalloproteases / biosynthesis*
  • Models, Biological*

Substances

  • DNA, Recombinant
  • Escherichia coli Proteins
  • Metalloproteases