NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes

Nat Methods. 2011 Oct 23;8(12):1037-40. doi: 10.1038/nmeth.1740.

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / metabolism
  • Biomarkers / analysis
  • Cell Differentiation
  • Cell Separation / methods*
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism*
  • Gene Expression Profiling
  • Green Fluorescent Proteins / metabolism*
  • Homeobox Protein Nkx-2.5
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Humans
  • Myoblasts, Cardiac / cytology*
  • Myoblasts, Cardiac / metabolism
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism
  • Polymerase Chain Reaction
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Vascular Cell Adhesion Molecule-1 / genetics
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • Antigens, Differentiation
  • Biomarkers
  • Homeobox Protein Nkx-2.5
  • Homeodomain Proteins
  • NKX2-5 protein, human
  • Receptors, Immunologic
  • SIRPA protein, human
  • Transcription Factors
  • Vascular Cell Adhesion Molecule-1
  • Green Fluorescent Proteins