Evaluation of vertical cell fluidity in a multilayered sheet of skeletal myoblasts

Masahiro Kino-oka; Trung Xuan Ngo; Eiji Nagamori; Yasunori Takezawa; Yasuki Miyake; Yoshiki Sawa; Atsuhiro Saito; Tatsuya Shimizu; Teruo Okano; Masahito Taya

doi:10.1016/j.jbiosc.2011.09.001

Evaluation of vertical cell fluidity in a multilayered sheet of skeletal myoblasts

J Biosci Bioeng. 2012 Jan;113(1):128-31. doi: 10.1016/j.jbiosc.2011.09.001. Epub 2011 Oct 20.

Authors

Masahiro Kino-oka¹, Trung Xuan Ngo, Eiji Nagamori, Yasunori Takezawa, Yasuki Miyake, Yoshiki Sawa, Atsuhiro Saito, Tatsuya Shimizu, Teruo Okano, Masahito Taya

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. kino-oka@bio.eng.osaka-u.ac.jp

PMID: 22018737
DOI: 10.1016/j.jbiosc.2011.09.001

Abstract

The procedure for fabricating a multilayered cell sheet has been developed by combining multiple sheets using a thermo-responsive surface and stamp system. Confocal laser scanning microscopy revealed that the fluidity of a multilayered sheet of skeletal myoblasts could be estimated as vertical diffusivity and changed upon addition of dermal fibroblasts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Movement
Cells, Cultured
Fibroblasts / cytology*
Humans
Image Processing, Computer-Assisted
Microscopy, Confocal
Myoblasts, Skeletal / cytology*
Temperature
Tissue Engineering / methods*