Structure and gene cluster of the O-antigen of Escherichia coli O19ab

Andrei V Perepelov; Hongfei Zhu; Sof'ya N Senchenkova; Quan Wang; Alexander S Shashkov; Lei Wang; Yuriy A Knirel

doi:10.1016/j.carres.2011.09.013

Structure and gene cluster of the O-antigen of Escherichia coli O19ab

Carbohydr Res. 2011 Dec 13;346(17):2812-5. doi: 10.1016/j.carres.2011.09.013. Epub 2011 Sep 24.

Authors

Andrei V Perepelov¹, Hongfei Zhu, Sof'ya N Senchenkova, Quan Wang, Alexander S Shashkov, Lei Wang, Yuriy A Knirel

Affiliation

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru

PMID: 22015401
DOI: 10.1016/j.carres.2011.09.013

Abstract

The O-polysaccharide (O-antigen) of Escherichia coli O19ab was studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established: [See formula in text] where the degree of O-acetylation of GlcNAc is ∼33%. The O-antigen gene cluster of E. coli O19ab was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O19ab-antigen structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Escherichia coli / genetics*
Gene Order
Molecular Sequence Data
Multigene Family*
O Antigens / genetics*

Substances

O Antigens