Anti-inflammatory effects of fatty acids isolated from Chromolaena odorata

Asian Pac J Trop Med. 2011 Oct;4(10):760-3. doi: 10.1016/S1995-7645(11)60189-2.

Abstract

Objective: To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata (C. odorata).

Methods: The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells.

Results: Six fatty acids (S)-coriolic acid (1), (S)-coriolic acid methyl ester (2), (S)-15,16-didehydrocoriolic acid (3), (S)-15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide (6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-κB inhibition. Compound 2 was the most active with the IC(50) values of 5.22 and 5.73 μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities.

Conclusions: The fatty acid components in C. odorata with NF-κB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine. This study could also contribute to the better use of C. odorata for human health care.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Chromolaena / chemistry*
  • Fatty Acids / isolation & purification
  • Fatty Acids / pharmacology*
  • Macrophages / drug effects*
  • Mice
  • NF-kappa B / antagonists & inhibitors*
  • Nitric Oxide / antagonists & inhibitors*
  • Plant Components, Aerial / chemistry
  • Plant Extracts / pharmacology*

Substances

  • Fatty Acids
  • NF-kappa B
  • Plant Extracts
  • Nitric Oxide