Abstract
Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Bordetella / genetics
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Bordetella / isolation & purification*
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Bordetella Infections / diagnosis*
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Bordetella Infections / epidemiology*
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Bordetella Infections / microbiology
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Child
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Child, Preschool
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DNA Transposable Elements
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification*
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France
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Humans
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Middle Aged
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Nasopharynx / microbiology*
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Prevalence
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Real-Time Polymerase Chain Reaction
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Retrospective Studies
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Young Adult
Substances
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DNA Transposable Elements
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DNA, Bacterial