Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis

J Clin Microbiol. 2011 Dec;49(12):4347-8. doi: 10.1128/JCM.01272-11. Epub 2011 Oct 19.

Abstract

Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Bordetella / genetics
  • Bordetella / isolation & purification*
  • Bordetella Infections / diagnosis*
  • Bordetella Infections / epidemiology*
  • Bordetella Infections / microbiology
  • Child
  • Child, Preschool
  • DNA Transposable Elements
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • France
  • Humans
  • Middle Aged
  • Nasopharynx / microbiology*
  • Prevalence
  • Real-Time Polymerase Chain Reaction
  • Retrospective Studies
  • Young Adult

Substances

  • DNA Transposable Elements
  • DNA, Bacterial