Objective: To construct a new 3D porous bone substitute material with collagen, hydroxyapatite and chondroitin sulfate, which has the main components of nature bone and the cell growth factor BMP-2 with bone inductive ability.
Methods: Collagen-hydroxyapatite-chondroitin sulfate scaffolds were prepared by chemical cross linking and freeze-drying, and bone morphogenetic protein (BMP) was incorporated into the scaffolds by adsorption. The bone substitute material was investigated by HE analysis, scanning electron microscope(SEM), electron spectroscopy for chemical analysis(ESCA), and X-ray diffraction(XRD). Rat mesenchymal stem cells (MSCs) were seeded into the scaffolds and cultured to form cell/scaffold (CS) constructs in vitro. The ectopic osteoinduction of the scaffolds were evaluated in vivo.
Results: The bone substitute material had a porous 3D structure facilitating cells growing into it. Implanted into the muscle, the scaffolds were degraded with the forming of new bone.
Conclusion: Our Findings indicate that the bone substitute material has good biocompatibility and its attachment to CS could improve the adhesion and differentiation of cells.