[Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS]

Minxia Zheng; Zhe Chen; Pei Liu; Yuqing Ge; Qun Dai

[Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS]

Zhongguo Zhong Yao Za Zhi. 2011 Jun;36(12):1641-3.

[Article in Chinese]

Authors

Minxia Zheng¹, Zhe Chen, Pei Liu, Yuqing Ge, Qun Dai

Affiliation

¹ National Clinical Research Base of Traditional Chinese Medicine, Zhejiang Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.

PMID: 22007552

Abstract

Objective: To investigate the monoterpene glycosides in Paeonia lactflora by UPLC-MS/MS.

Method: An Acquity UPLC BEH C18 column (2.1 mm x 50 mm) with 1.7 microm particle size was used. The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode. The flow rate was 0.4 mL x min and the chromatographic run time was 9 min for one run. The mass spectrometer equipped with an eletrospray ion source in negative ion mode.

Results: Totally six glycosides were analyzed and identified by the established UPLC-MS/MS method.

Conclusion: The method was rapid, sensitive, and extremely useful for rapid identification of glycosides in P. lactiflora.

Publication types

English Abstract

MeSH terms

Molecular Structure
Paeonia / chemistry*
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization / methods*
Tandem Mass Spectrometry / methods*