Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes

Immunol Lett. 1990 Jun;24(3):165-9. doi: 10.1016/0165-2478(90)90043-p.

Abstract

A 1.9-kb fragment containing an interleukin-8 (IL-8) coding region was amplified by the polymerase chain reaction (PCR) from the genomic DNA of human lung giant cell carcinoma LU65C cells that produce LUCT/IL-8 with N-terminal sequence of AVLPR. The coding region was found to consist of 4 exons and 3 introns as identical as that of the gene of MDNCF/IL-8 lacking N-terminal AVLPR. PCR using genomic DNAs from human polymorphonuclear leukocytes and mononuclear cells also provided the same 1.9-kb fragment as that from LU65C genomic DNA. Thus, it seems likely that human cells possess IL-8 genes with the homogeneous coding region so that they may first produce the same mature protein with N-terminal AVLPR (= LUCT) which was then truncated.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carcinoma / genetics*
  • Carcinoma / pathology
  • Chemotactic Factors / genetics*
  • DNA, Neoplasm / genetics
  • Genes*
  • Humans
  • Interleukin-8
  • Interleukins / genetics*
  • Leukocytes / analysis
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Polymerase Chain Reaction
  • Protein Processing, Post-Translational*
  • Sequence Homology, Nucleic Acid
  • Tumor Cells, Cultured / analysis

Substances

  • Chemotactic Factors
  • DNA, Neoplasm
  • Interleukin-8
  • Interleukins
  • Neoplasm Proteins