Enzyme electrode for aromatic compounds exploiting the catalytic activities of microperoxidase-11

Biosens Bioelectron. 2011 Dec 15;30(1):320-3. doi: 10.1016/j.bios.2011.09.004. Epub 2011 Sep 16.

Abstract

Microperoxidase-11 (MP-11) which has been immobilised in a matrix of chitosan-embedded gold nanoparticles on the surface of a glassy carbon electrode catalyzes the conversion of aromatic substances. This peroxide-dependent catalysis of microperoxidase has been applied in an enzyme electrode for the first time to indicate aromatic compounds such as aniline, 4-fluoroaniline, catechol and p-aminophenol. The electrode signal is generated by the cathodic reduction of the quinone or quinoneimine which is formed in the presence of both MP-11 and peroxide from the substrate. The same sensor principle will be extended to aromatic drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Catalysis
  • Conductometry / instrumentation*
  • Enzyme Activation
  • Enzymes, Immobilized / chemistry
  • Equipment Design
  • Equipment Failure Analysis
  • Hydrocarbons, Aromatic / analysis*
  • Oligopeptides / chemistry*

Substances

  • Enzymes, Immobilized
  • Hydrocarbons, Aromatic
  • MP 11
  • Oligopeptides