Genetic variation in salt (Na(2)SO(4), NaCl) and boron (B) tolerance among four ecotypes of the selenium (Se) hyperaccumulator Stanleya pinnata (Pursh) Britton was utilized to select tolerant genotypes capable of phytoremediating Se from salt, B, and Se-laden agricultural drainage sediment. The few individual salt/B tolerant genotypes were successfully selected from among a large population of highly salt/B sensitive seedlings. The distribution, hyperaccumulation, and volatilization of Se were then examined in selected plants capable of tolerating the high salt/B laden drainage sediment. Salt/B tolerant genotypes from each of the four ecotypes had mean Se concentrations ranging from 2510 ± 410 to 1740 ± 620 in leaves and 3180 ± 460 to 2500 ± 1060 in seeds (μg Se g(-1) DW ± SD), while average daily Se volatilization rates ranged from 722 ± 375 to 1182 ± 575 (μg Se m(-2) d(-1) ± SD). After two growing seasons (∼18 months), we estimated that hyperaccumulation and volatilization of Se by tolerant S. pinnata genotypes and their associated microbes can remove approximately 30% of the total soil Se in 0-30 cm sediment. The salt/B tolerant S. pinnata genotypes selected and characterized herein represent promising new tools for the successful phytoremediation of Se from salt/B and Se-laden agricultural drainage sediments.