A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties

Holly A Reeve; Lars Lauterbach; Philip A Ash; Oliver Lenz; Kylie A Vincent

doi:10.1039/c1cc14826e

A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties

Chem Commun (Camb). 2012 Feb 1;48(10):1589-91. doi: 10.1039/c1cc14826e. Epub 2011 Oct 10.

Authors

Holly A Reeve¹, Lars Lauterbach, Philip A Ash, Oliver Lenz, Kylie A Vincent

Affiliation

¹ Inorganic Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3QR, UK.

PMID: 21986817
DOI: 10.1039/c1cc14826e

Abstract

Pyrolytic graphite particles modified with hydrogenase and an NAD(+)/NADH cycling enzyme provide a modular heterogeneous catalyst system for regeneration of oxidised or reduced nicotinamide cofactors using H(2) and H(+) as electron source or sink. Particles can be tuned for cofactor supply under different conditions by appropriate choice of hydrogenase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis
Graphite / chemistry
Graphite / metabolism*
Hydrogenase / chemistry
Hydrogenase / metabolism*
NAD / chemistry
NAD / metabolism*
NADPH Dehydrogenase / chemistry
NADPH Dehydrogenase / metabolism*
Oxidation-Reduction
Particle Size

Substances

NAD
Graphite
Hydrogenase
NADPH Dehydrogenase