Objective: To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring.
Design: Randomized research experimental study.
Setting: Academic basic research laboratory.
Animal(s): Mice.
Intervention(s): Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring.
Main outcome measure(s): Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19.
Result(s): Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3.
Conclusion(s): Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring.
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.