Abstract
The purpose of this study was to prepare a propyl gallate (PrG) molecular imprinted polymer as a cartridge stuffing material to isolate antiplatelet active ingredients. A macroporous polymer was synthesized utilizing ethylene glycol dimethacrylate (EDMA) as the crosslinking agent, PrG as the template molecule and 4-vinylpyridine (4-Vpy) as the functional monomer. Subsequently, PrG was removed by washing with methanol-glacial acetic acid (9:1, v/v). The molecular imprinted polymer recognized an active ingredient, protocatechuic acid, from a crude extract of the Chinese herbal medicine, Radix Salviae Miltiorrhizae (Danshen), using an on-line column switching solid phase extraction process. Pharmacological experiments showed that protocatechuic acid inhibits arachidonic acid (10 mg/kg) induced aggregation of rat platelets in vivo. This study provides an example of an application of separation-analysis technique for screening potentially bioactive compounds.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonic Acid / antagonists & inhibitors
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Arachidonic Acid / pharmacology
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Blood Platelets / drug effects
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Chromatography, Liquid / methods
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Drug Interactions
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Drugs, Chinese Herbal / chemistry*
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Drugs, Chinese Herbal / pharmacology
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Hydroxybenzoates / chemistry*
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Hydroxybenzoates / pharmacology*
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Male
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Methacrylates / chemistry
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Molecular Imprinting / methods
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Phenanthrolines / chemistry*
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Phenanthrolines / pharmacology
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Platelet Aggregation / drug effects
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Platelet Aggregation Inhibitors / chemistry*
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Platelet Aggregation Inhibitors / pharmacology
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Polymerization
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Polymers / chemistry*
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Propyl Gallate / chemistry
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Pyridines / chemistry
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Random Allocation
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Rats
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Rats, Wistar
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Salvia miltiorrhiza / chemistry*
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Solid Phase Extraction / methods
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Tandem Mass Spectrometry / methods
Substances
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Drugs, Chinese Herbal
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Hydroxybenzoates
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Methacrylates
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Phenanthrolines
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Platelet Aggregation Inhibitors
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Polymers
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Pyridines
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dan-shen root extract
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Arachidonic Acid
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protocatechuic acid
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ethylene dimethacrylate
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Propyl Gallate
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4-vinylpyridine