Abstract
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo kinase and xylose isomerase. pXCH allows tightly regulated expression of the chloramphenicol acetyltransferase reporter and the α-toxin genes in response to the inducer concentration. Thus, pXCH could constitute a new valuable genetic tool for study of C. perfringens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldose-Ketose Isomerases / genetics
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Aldose-Ketose Isomerases / metabolism
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Bacterial Toxins / biosynthesis
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Bacterial Toxins / genetics
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Calcium-Binding Proteins / biosynthesis
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Calcium-Binding Proteins / genetics
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / genetics
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Clostridioides difficile / genetics
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Clostridium perfringens / genetics*
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Clostridium perfringens / metabolism*
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Gene Expression Regulation, Bacterial*
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Gene Expression*
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Genes, Reporter
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Genetic Engineering / methods
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Genetic Vectors*
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Genetics, Microbial / methods*
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Operator Regions, Genetic
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Plasmids
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Promoter Regions, Genetic
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Repressor Proteins / genetics
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Type C Phospholipases / biosynthesis
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Type C Phospholipases / genetics
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Xylose / metabolism*
Substances
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Bacterial Toxins
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Calcium-Binding Proteins
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Repressor Proteins
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Xylose
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Chloramphenicol O-Acetyltransferase
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Phosphotransferases (Alcohol Group Acceptor)
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xylulokinase
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Type C Phospholipases
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alpha toxin, Clostridium perfringens
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Aldose-Ketose Isomerases
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xylose isomerase