Synthesis of new Galβ1→4Fuc segments useful for biological investigations

Chem Pharm Bull (Tokyo). 2011;59(10):1307-10. doi: 10.1248/cpb.59.1307.

Abstract

Useful segments (1, 2) for chemical probes embedded in a Galβ1→4Fuc unit were designed and prepared for characterizing sugar-binding proteins in Caenorhabditis elegans. Segment 1 with an amino group terminus was used as a recognition unit in affinity chromatography. It was revealed that some proteins (annexins and galectins) in C. elegans have an affinity for Galβ1→4Fuc.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins*
  • Chromatography, Affinity
  • Fucose / chemistry*
  • Galactose / chemistry*
  • Galectins / chemistry
  • Galectins / metabolism
  • Molecular Probes / analysis
  • Molecular Probes / chemical synthesis*
  • Molecular Probes / chemistry*
  • Molecular Probes / metabolism
  • Oligosaccharides / chemistry*
  • Oligosaccharides / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Galectins
  • Molecular Probes
  • Oligosaccharides
  • Fucose
  • Galactose