Abstract
Sauchinone, a biologically active lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to a reduction in COX-2-derived prostaglandin E(2) (PGE(2)) and iNOS-derived nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. Present study also demonstrates the effects of sauchinone in inducing heme oxygenase-1 (HO-1) expression and an increase in heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sauchinone on LPS-induced PGE(2), NO, tumor necrosis factor-α (TNF-α) and interlukine-1β (IL-1β) production were partially reversed by the HO-1 inhibitor Tin protoporphyrin was also seen in this study. In addition, we found that treatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) reduced sauchinone-induced HO-1 expression. Sauchinone also increased ERK phosphorylation. These results suggest that sauchinone inhibits pro-inflammatory mediators through expression of anti-inflammatory HO-1 via ERK pathway.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Inflammatory Agents / chemistry
-
Anti-Inflammatory Agents / immunology
-
Anti-Inflammatory Agents / metabolism
-
Anti-Inflammatory Agents / pharmacology*
-
Benzopyrans / chemistry
-
Benzopyrans / immunology
-
Benzopyrans / metabolism
-
Benzopyrans / pharmacology*
-
Cell Line
-
Cyclooxygenase 2 / immunology
-
Cyclooxygenase 2 / metabolism
-
Dioxoles / chemistry
-
Dioxoles / immunology
-
Dioxoles / metabolism
-
Dioxoles / pharmacology*
-
Drug Evaluation, Preclinical
-
Enzyme Inhibitors / metabolism
-
Enzyme Inhibitors / pharmacology
-
Extracellular Signal-Regulated MAP Kinases / drug effects
-
Extracellular Signal-Regulated MAP Kinases / immunology
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Flavonoids / metabolism
-
Flavonoids / pharmacology
-
Heme Oxygenase-1 / immunology
-
Heme Oxygenase-1 / metabolism
-
Inflammation / drug therapy*
-
Inflammation / physiopathology
-
Inflammation Mediators / antagonists & inhibitors*
-
Inflammation Mediators / immunology
-
Lipopolysaccharides / immunology
-
Lipopolysaccharides / pharmacology
-
Macrophages / drug effects
-
Macrophages / immunology
-
Metalloporphyrins
-
Mice
-
Molecular Targeted Therapy
-
Nitric Oxide / biosynthesis
-
Nitric Oxide / immunology
-
Nitric Oxide Synthase Type II / antagonists & inhibitors
-
Nitric Oxide Synthase Type II / drug effects
-
Phosphorylation / drug effects
-
Phytotherapy
-
Plant Preparations / chemistry
-
Plant Preparations / isolation & purification
-
Plant Preparations / pharmacology*
-
Plant Roots
-
Protoporphyrins
-
Saururaceae*
-
Signal Transduction / drug effects
-
Tumor Necrosis Factor-alpha / antagonists & inhibitors
-
Tumor Necrosis Factor-alpha / biosynthesis
-
Tumor Necrosis Factor-alpha / immunology
Substances
-
Anti-Inflammatory Agents
-
Benzopyrans
-
Dioxoles
-
Enzyme Inhibitors
-
Flavonoids
-
Inflammation Mediators
-
Lipopolysaccharides
-
Metalloporphyrins
-
Plant Preparations
-
Protoporphyrins
-
Tumor Necrosis Factor-alpha
-
sauchinone
-
Nitric Oxide
-
tin protoporphyrin IX
-
NOS2 protein, human
-
Nitric Oxide Synthase Type II
-
Heme Oxygenase-1
-
Cyclooxygenase 2
-
Extracellular Signal-Regulated MAP Kinases
-
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one