Abstract
Background:
Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.
Results:
We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.
Conclusions:
Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
Publication types
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Antineoplastic Agents, Alkylating / pharmacology
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism
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Apoptosis*
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Arachidonic Acid / metabolism*
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Cell Line, Tumor
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Dacarbazine / analogs & derivatives
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Dacarbazine / pharmacology
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Docosahexaenoic Acids / metabolism*
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Down-Regulation / drug effects
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Glioblastoma / drug therapy
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Glioblastoma / metabolism
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Glioma / drug therapy
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Glioma / metabolism*
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Humans
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism
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Osmolar Concentration
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RNA, Messenger / metabolism
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Temozolomide
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Up-Regulation* / drug effects
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gamma-Linolenic Acid / metabolism*
Substances
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Antineoplastic Agents, Alkylating
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Apoptosis Regulatory Proteins
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MicroRNAs
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Neoplasm Proteins
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RNA, Messenger
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Docosahexaenoic Acids
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Arachidonic Acid
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gamma-Linolenic Acid
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Dacarbazine
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Temozolomide