The adoption of principles of evidence-based practice in nursing education assists nursing graduates in improving their practice. Measuring factors that influence this adoption requires the use of a valid tool; however, few tools addressing these factors are available. This study measured the psychometric properties of a composite tool developed to assess factors influencing the adoption of the principles of evidence-based practice in nursing education. A descriptive, cross-sectional research design was used. A convenience, purposive sample of 85 nurse educators participated in this study. The Evidence-Based Nursing Education Questionnaire items were derived following a four-step approach: extensive literature review, thematic analysis of literature, expert opinion in the questionnaire items, and psychometric testing of the questionnaire. The psychometric properties indicated a valid and reliable tool. The resulting five factors include knowledge in educational principles and faculty practice. The questionnaire proved to be reliable and valid.