Cross-talk between human mesenchymal stem/progenitor cells (MSCs) and rat hippocampal slices in LPS-stimulated cocultures: the MSCs are activated to secrete prostaglandin E2

J Neurochem. 2011 Dec;119(5):1052-63. doi: 10.1111/j.1471-4159.2011.07511.x. Epub 2011 Nov 1.

Abstract

Mesenchymal stem/progenitor cells (MSCs) improve functional outcome in a number of disease models through suppression of inflammation. However, their effects on neuroinflammation are unknown. In this study, we show that MSCs suppress endotoxin-induced glial activation in organotypic hippocampal slice cultures (OHSCs). Lipopolysaccharide-stimulated OHSCs activated MSCs to increase the expression of cyclo-oxygenase-2 and produce prostaglandin E2. MSC-derived prostaglandin E2, then suppressed pro-inflammatory cytokine production by the OHSCs. Together, the results suggest the potential anti-inflammatory mechanism of MSCs in models of disease and support earlier observations that MSCs may offer a therapy for neuroinflammation produced by trauma or disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Communication / physiology*
  • Coculture Techniques
  • Cyclooxygenase 2 / metabolism
  • Cyclooxygenase 2 / physiology
  • Cytokines / biosynthesis
  • Cytokines / physiology
  • Dinoprostone / metabolism*
  • Female
  • Gliosis / metabolism*
  • Gliosis / pathology*
  • Hippocampus / cytology
  • Hippocampus / physiology*
  • Humans
  • Inflammation Mediators / physiology*
  • Lipopolysaccharides / physiology
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Neuroglia / cytology
  • Neuroglia / metabolism*
  • Organ Culture Techniques
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Cytokines
  • Inflammation Mediators
  • Lipopolysaccharides
  • Cyclooxygenase 2
  • Dinoprostone