Abstract
As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1-3, 3-5, and 5-10 kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3-5 kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3-5 kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3-5 kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Survival / drug effects
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Cells, Cultured
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Chitosan / analogs & derivatives*
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Collagen / metabolism
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DNA Damage / drug effects*
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Fibroblasts / radiation effects
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Gene Expression / drug effects
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Humans
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L-Lactate Dehydrogenase / metabolism
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Matrix Metalloproteinase 1 / genetics
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Matrix Metalloproteinase 1 / metabolism
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Matrix Metalloproteinase 13 / genetics
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Matrix Metalloproteinase 13 / metabolism
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Mitogen-Activated Protein Kinases / metabolism
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Oligosaccharides / pharmacology*
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Radiation-Protective Agents / pharmacology*
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Reactive Oxygen Species / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Skin / cytology
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Ultraviolet Rays*
Substances
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Oligosaccharides
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Radiation-Protective Agents
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Reactive Oxygen Species
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Collagen
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Chitosan
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L-Lactate Dehydrogenase
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Mitogen-Activated Protein Kinases
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MMP13 protein, human
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Matrix Metalloproteinase 13
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MMP1 protein, human
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Matrix Metalloproteinase 1