Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)

Am J Bot. 2011 Oct;98(10):e284-6. doi: 10.3732/ajb.1100221. Epub 2011 Sep 22.

Abstract

Premise of the study: Microsatellite loci were developed in Tsoongiodendron odorum, an endangered Magnoliaceae species in subtropical China, for further investigation of its conservation genetics.

Methods and results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, we isolated and characterized 12 microsatellite loci from T. odorum. Of the samples analyzed, eight were polymorphic and four were monomorphic.

Conclusions: These characterized markers will enable genetic diversity, gene flow, and mating system studies of T. odorum, which are highly valuable for formulating conservation strategies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Plant / genetics*
  • DNA, Plant / isolation & purification*
  • Genetic Loci / genetics*
  • Magnoliaceae / genetics*
  • Microsatellite Repeats / genetics*
  • Molecular Sequence Data
  • Polymorphism, Genetic

Substances

  • DNA, Plant

Associated data

  • GENBANK/JF916893
  • GENBANK/JF916894
  • GENBANK/JF916895
  • GENBANK/JF916896
  • GENBANK/JF916897
  • GENBANK/JF916898
  • GENBANK/JF916899
  • GENBANK/JF916900
  • GENBANK/JF916901
  • GENBANK/JF916902
  • GENBANK/JF916903
  • GENBANK/JF916904