DNA microarray genotyping and virulence and antimicrobial resistance gene profiling of methicillin-resistant Staphylococcus aureus bloodstream isolates from renal patients

Sinead McNicholas; Anna C Shore; David C Coleman; Hilary Humphreys; Deirdre Fitzgerald Hughes

doi:10.1128/JCM.05017-11

DNA microarray genotyping and virulence and antimicrobial resistance gene profiling of methicillin-resistant Staphylococcus aureus bloodstream isolates from renal patients

J Clin Microbiol. 2011 Dec;49(12):4349-51. doi: 10.1128/JCM.05017-11. Epub 2011 Sep 21.

Authors

Sinead McNicholas¹, Anna C Shore, David C Coleman, Hilary Humphreys, Deirdre Fitzgerald Hughes

Affiliation

¹ Department of Clinical Microbiology, RCSI Education and Research Centre, Smurfit Building, Beaumont Hospital, Dublin 9, Ireland.

Abstract

Thirty-six methicillin-resistant Staphylococcus aureus (MRSA) bloodstream isolates from renal patients were genetically characterized by DNA microarray analysis and spa typing. The isolates were highly clonal, belonging mainly to ST22-MRSA-IV. The immune evasion and enterotoxin gene clusters were found in 29/36 (80%) and 33/36 (92%) isolates, respectively.

MeSH terms

Aged
Bacteremia / microbiology*
Drug Resistance, Bacterial*
Female
Humans
Male
Methicillin-Resistant Staphylococcus aureus / classification
Methicillin-Resistant Staphylococcus aureus / drug effects
Methicillin-Resistant Staphylococcus aureus / genetics*
Methicillin-Resistant Staphylococcus aureus / pathogenicity
Microarray Analysis
Molecular Typing
Oligonucleotide Array Sequence Analysis / methods*
Renal Dialysis / adverse effects
Staphylococcal Infections / microbiology*
Virulence
Virulence Factors / genetics*

Substances

Virulence Factors