A fractal-like kinetic equation to investigate temperature effect on cellulose hydrolysis by free and immobilized cellulase

Appl Biochem Biotechnol. 2012 Sep;168(1):144-53. doi: 10.1007/s12010-011-9362-4. Epub 2011 Sep 21.

Abstract

According to fractal-like theory in the heterogeneous system, a cellulase-catalyzed kinetic equation that contained two parameters (rate constant k and fractal dimension h) was deduced. The equation described directly the mathematical relationship between reducing sugar concentration and hydrolytic time, and accurately fitted the experimental data of free/immobilized cellulase at 37, 40, 44, 47, and 50 °C (R(2)>0.99). The fitted h value is estimated as a constant (0.6148) in these tested temperatures. The fitted k value increased with temperature increase, and the relationship agreed with Arrhenius equation (R(2)>0.98). The fractal-like equation could predict accurately the experimental data at low temperature 34 °C for free/immobilized cellulase and high temperature 53 °C for immobilized cellulase, but the prediction at 53 °C for free cellulase was not accurate enough due to its lower stability than immobilized cellulase. The application of fractal-like theory in cellulase kinetics is successful.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkalies / chemistry
  • Carbohydrates / analysis
  • Cellulase / metabolism*
  • Cellulose / metabolism*
  • Enzymes, Immobilized / metabolism
  • Fractals*
  • Hydrolysis
  • Kinetics
  • Models, Biological
  • Oxidation-Reduction
  • Regression Analysis
  • Temperature*

Substances

  • Alkalies
  • Carbohydrates
  • Enzymes, Immobilized
  • Cellulose
  • Cellulase