Abstract
A cobalt-phosphine complex supported on PS-PEG beads was found to react with a propargyl carbamate tag, and the tagged molecules immobilized on the beads could be released by acidic treatment through the Nicholas reaction pathway. These reactions work in aqueous media at 4 °C, so that this catch and release procedure is compatible with conditions generally used in biochemical experiments.
MeSH terms
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Alkynes / chemistry*
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Avidin / chemistry
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Avidin / metabolism
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Biotin / chemistry
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Biotin / metabolism
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Chemical Fractionation / methods*
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Chemistry, Organic / methods*
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Click Chemistry / methods*
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Cobalt / chemistry
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Copper / chemistry
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Phosphines / chemistry
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Polyethylene Glycols / chemistry
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Polystyrenes / chemistry
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Solubility
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Spectroscopy, Fourier Transform Infrared
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Water
Substances
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Alkynes
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Phosphines
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Polystyrenes
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polyethylene glycol-polystyrene
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Water
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Avidin
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Cobalt
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Polyethylene Glycols
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Biotin
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Copper