A convenient preparation of multi-spectral microparticles by bacteria-mediated assemblies of conjugated polymer nanoparticles for cell imaging and barcoding

Xuli Feng; Gaomai Yang; Libing Liu; Fengting Lv; Qiong Yang; Shu Wang; Daoben Zhu

doi:10.1002/adma.201102026

A convenient preparation of multi-spectral microparticles by bacteria-mediated assemblies of conjugated polymer nanoparticles for cell imaging and barcoding

Adv Mater. 2012 Feb 2;24(5):637-41. doi: 10.1002/adma.201102026. Epub 2011 Sep 20.

Authors

Xuli Feng¹, Gaomai Yang, Libing Liu, Fengting Lv, Qiong Yang, Shu Wang, Daoben Zhu

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.

PMID: 21932281
DOI: 10.1002/adma.201102026

Abstract

A novel technique was developed for preparing encoded multicolour microparticles based on the self-assembly of bacteria and conjugated polymer nanoparticles (CPNs) by a very simple and time-saving manner. These bacteria-CPNs microparticles show multicolor emissions by tuning FRET efficiencies among CPNs under single excitation wavelength and can be successfully applied for cell imaging and optical barcoding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Escherichia coli / cytology
Flow Cytometry
Fluorescent Dyes / chemistry*
Humans
Microscopy, Fluorescence / methods*
Nanoparticles / chemistry*
Polymers / chemistry*

Substances

Fluorescent Dyes
Polymers