Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed

Oscar Castillo-Fernandez; Georgette B Salieb-Beugelaar; Jan W van Nieuwkasteele; Johan G Bomer; Martin Arundell; Josep Samitier; Albert van den Berg; Jan C T Eijkel

doi:10.1002/elps.201100278

Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed

Electrophoresis. 2011 Sep;32(18):2402-9. doi: 10.1002/elps.201100278. Epub 2011 Aug 26.

Authors

Oscar Castillo-Fernandez¹, Georgette B Salieb-Beugelaar, Jan W van Nieuwkasteele, Johan G Bomer, Martin Arundell, Josep Samitier, Albert van den Berg, Jan C T Eijkel

Affiliation

¹ Nanobioengineering Group, Institute for Bioengineering of Catalonia, Josep Samitier, Barcelona, Spain.

PMID: 21922490
DOI: 10.1002/elps.201100278

Abstract

The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Bacteriophage lambda / chemistry
Bacteriophage lambda / genetics
DNA, Viral / analysis
DNA, Viral / chemistry*
Electrophoresis / instrumentation*
Electrophoresis / methods
Microfluidic Analytical Techniques / instrumentation*
Microfluidic Analytical Techniques / methods
Polymers
Silicon Dioxide / chemistry
Statistics, Nonparametric

Substances

DNA, Viral
Polymers
Silicon Dioxide