Fluorescence microscopy of nanochannel-confined DNA

Fredrik Persson; Fredrik Westerlund; Jonas O Tegenfeldt

doi:10.1007/978-1-61779-282-3_9

Fluorescence microscopy of nanochannel-confined DNA

Methods Mol Biol. 2011:783:159-79. doi: 10.1007/978-1-61779-282-3_9.

Authors

Fredrik Persson¹, Fredrik Westerlund, Jonas O Tegenfeldt

Affiliation

¹ Department of Physics, University of Gothenburg, Gothenburg, Sweden.

PMID: 21909888
DOI: 10.1007/978-1-61779-282-3_9

Abstract

Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Microscopy, Fluorescence / methods*
Nanostructures / chemistry*
Nanotechnology

Substances

DNA